mouse spontaneous mammary adenocarcinoma as a suitable model of breast cancer in iran

background : breast cancer is a complicated disease and has various clinical manifestations and prognosis. the aim of this study is to make a breast cancer model suitable for basic, preclinical and clinical studies. materials and methods: five virgin bulb/c mice aged 6 weeks and spontaneous murine mammary adenocarcinoma cells (mmac) were used. after anesthesia induction, 1-3 million of mmacs were subcutaneously injected into the mice’s right flank. four weeks after the tumor induction, tumor size in both length and width were weekly measured using caliper for 4 weeks. histopathology studies were done 2 months later by scraff- boom-richardson grading. results: visible tumors were appeared after 4 weeks. take-rate of all cells was nearly 100%. tumor sizes reached 0.6â±0.1 mm 3 , 1.1â±0.3, 1.68â±0.5 and 3â±0.7 cm 3 with at fifth, sixth, seventh and eighth weeks respectively. the histopathological studies using h & e method showed invasive ductal carcinoma, grade ii/iii. conclusions: elucidation of the molecular mechanisms of breast cancer progression and metastasis has extremely gained from mouse models in which some stages of tumor progression are recapitulated.